NHS Blood and Transplant (NHSBT) estimate that the demand for blood type O- will surge by 70% during the 2012 Games.
Statistics show that during public holidays, demand for type O- blood - which can be transfused into any patient regardless of their own blood type - rises significantly.
During the Diamond Jubilee celebrations, demand was 69% higher, statistics show.
NHSBT urged donors, including those with other blood types, to come forward ahead of an "exceptionally busy" summer for blood banks.
Spokesman Jon Latham said: "Without O- blood donors, many live saving procedures could be delayed or made more risky for patients and of course we wouldn't be able to give transfusions to people with O- at all since they can only take blood of their own type.
"We're appealing to this elite group of blood donors to help us prepare for the unprecedented demand expected around the Olympics to make sure our health services have the essential stocks they need."
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